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KMID : 0894520070110020079
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Development & Reproduction
2007 Volume.11 No. 2 p.79 ~ p.84
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The Effects of Cryopreservation on Fine Structures of Pearl Oyster (Pinctada fucata martensii) Larvae
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Choi Youn-Hee
Jo Pil-Gue
Kim Tae-ik
Bai Sung-chul
Chang Young-Jin
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Abstract
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The freezing susceptibilities of two larval stages (D-shaped and umbo) of the pearl oyster (Pinctada fucata martensii) were evaluated by the electron microscopy (light, transmission electron and scanning electron). The morphological shapes were examined from each pre-frozen or frozen-thawed stage of the cryopreserved larvae in liquid nitrogen by using the cryoprotectant, dimethyl sulfoxide (Me₂SO) mixed with sucrose. Although a portion of the shell was damaged, the hinge and prodissoconch were intact and clearly visible after preservation in liquid nitrogen. In addition, the cytoplasm of the frozen-thawed larvae maintained the normal organelle integrities, e.g., endoplasmic reticula, lipid droplets, mitochondria, nucleus and microvilli. However, some of the frozen-thawed larvae showed irregularly arranged cilia, rough shell surfaces and round-lumped cilium heads. These results indicate that P. fucata martensii larvae are susceptible to freezing, at least at those two critical developmental stages (D-shaped and umbo), and suggest a new industrial investigation including reduction method of cell injury for preserving microbial starter cultures need to be developed.
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KEYWORD
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Pearl oyster, Pinctada fucata martensii, Larvae, Cryopreservation, Fine structure
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